Novel bead-based platform for direct detection of unlabelled nucleic acids through Single Nucleobase Labelling

A molecular beacon, bead-based assay for the detection of nucleic acids by flow cytometry

Molecular beacons are dual-labelled probes that are typically used in real-time PCR assays, but have also been conjugated with solid matrices for use in microarrays or biosensors. We have developed a fluid array system using microsphere-conjugated molecular beacons and the flow cytometer for the specific, multiplexed detection of unlabelled nucleic acids in solution. For this array system, mole...

Au nanoparticles/g-C3N4 modified biosensor for electrochemical detection of gastric cancer miRNA based on hairpin locked nucleic acids probe

Objective: The annual incidence of cancer in the world is growing rapidly. The most important factor in the cure of cancers is their early diagnosis. miRNA, as a biomarker for early detection of cancer, has attracted a lot of attention. Methods: In this study, an electrochemical biosensor was developed to detect the amount of miR-106a, the biomarker of gastric cancer, by modifying a glass...

Click chemistry facilitates direct labelling and super-resolution imaging of nucleic acids and proteins

Various uorescence microscopy techniques which can bypass the spatial resolution limit have been developed in recent years. Commonly summarized as “super-resolution microscopy” techniques, they constitute a new and valuable toolbox for cell biology (for details on the different techniques, we refer to some recent reviews). All uorescence microscopy-based methods require suitable and specic s...

Detection of single base changes in nucleic acids.

Novel Biochip Platform for Nucleic Acid Analysis

This manuscript describes the use of a novel biochip platform for the rapid analysis/identification of nucleic acids, including DNA and microRNAs, with very high specificity. This approach combines a unique dynamic chemistry approach for nucleic acid testing and analysis developed by DestiNA Genomics with the STMicroelectronics In-Check platform, which comprises two microfluidic optimized and i...